Journal: PLOS ONE
Article Title: The PreQuine Platform: A novel diagnostic tool for measuring glucose-6-phosphate dehydrogenase (G6PD) activity and hemoglobin concentration
doi: 10.1371/journal.pone.0297918
Figure Lengend Snippet: a) Exploded view of the G6PD dual analyte test strip. (1) a spreading layer (transport membrane), (2) a G6PD reagent membrane (hollow fiber membrane with electron mediator, inhibitor, and substrate), (3) a G6PD detection membrane (mixed mesh < 1 μm) facing the LED, (4) a reagent membrane (polyether sulfone membrane containing sodium azide and sodium nitrite) and (5) an optical membrane (polyether sulfone membrane < 1 μm) facing the LED. b) Top and bottom view of the fully assembled test strip with representative color change after test strip chemical reaction (far right). c) PreQuine Platform’s reflectance-based meter shows both 570 and 670 nm LEDs that are used for the simultaneous detection of Hgb and G6PD, respectively.
Article Snippet: G6PD enzyme activity was adjusted via freeze/thaw cycles and/or spiking with recombinant G6PD enzyme (Cat# 078A0020, Calzyme, San Luis Obispo, CA) while Hgb levels were adjusted through the addition and subtraction of plasma.
Techniques: Stripping Membranes, Membrane